Finally, we highlight how an evolutionary approach can be useful to understand current topics of medical interest.Parkinson's disease (PD) is one of the most common neurodegenerative movement disorders, for which there has been no effective treatments. To clarify the pathogenesis of PD, we constructed a competing endogenous RNA (ceRNA) network based on the genome-wide RNA sequencing data. It was found that 92 RNAs were differentially expressed, including 50 mRNAs, 25 miRNAs and 17 lncRNAs, based on which a ceRNA network was constructed and evaluated from 4 aspects of number of nodes, topological coefficients, closeness centrality and betweenness centrality. The functional annotation and enrichment analysis suggested that 6 functional modules, particularly the peripheral nervous system development and toxin metabolic process, dominated the development of PD. To validate the assumption, the gene set enrichment analysis (GSEA) was conducted basing on the genome-wide RNAs regardless whether they were differentially expressed or not. Consistently, the results revealed that dysregulation of MAG, HOXB3, MYRF and PLP1 led to metabolic disorders of sphingolipid and glutathione, which contributed to the pathogenesis of PD. Also, in-depth mining of previous literature confirmed a pivotal role of these dysregulated RNAs, which had been indicated to be potential diagnostic and therapeutic biomarkers of PD. Overall, we constructed a ceRNA network based on the dysregulated mRNAs, lncRNAs and miRNAs in PD, and the aberrant expression of MAG, HOXB3, MYRF and PLP1 caused metabolism disorder of sphingolipid and glutathione, and these genes are of great significance for the diagnosis and treatment of PD.The GABAergic system is thought to play an important role in the control of cognition and emotion, such as fear, and is related to the pathophysiology of psychiatric disorders. For example, the expression of the 67-kDa isoform of glutamate decarboxylase (GAD67), a GABA-producing enzyme, is downregulated in the postmortem brains of patients with major depressive disorder and schizophrenia. However, knocking out the Gad1 gene, which encodes GAD67, is lethal in mice, and thus, the association between Gad1 and cognitive/emotional functions is unclear. We recently developed Gad1 knockout rats and found that some of them can grow into adulthood. Here, we performed fear-conditioning tests in adult Gad1 knockout rats to assess the impact of the loss of Gad1 on fear-related behaviors and the formation of fear memory. In a protocol assessing both cued and contextual memory, Gad1 knockout rats showed a partial antiphase pattern of freezing during training and significantly excessive freezing during the contextual test compared with wild-type rats. However, Gad1 knockout rats did not show any synchronous increase in freezing with auditory tones in the cued test. On the other hand, in a contextual memory specialized protocol, Gad1 knockout rats exhibited comparable freezing behavior to wild-type rats, while their fear extinction was markedly impaired. These results suggest that GABA synthesis by GAD67 has differential roles in cued and contextual fear memory.The aim of this in-vitro study was to determine the antimicrobial capacity of a Berberis vulgaris plant extract on the bacteria being associated with caries including, Streptococcus mutans, S. sobrinus, S. sanguinis, S. salivaris and Lactobacillus rhamnosus.
Chlorhexidine 2% (CHX) mouthwash and ampicillin (10?μg/disk) were applied as positive control groups. Inhibition zone, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) related to stem, leaf and fruit of B. vulgaris plant were recorded for every five bacteria. Data were analyzed using SPSS ver. 22, one-way ANOVA repeated measure and post hoc Tukey statistical test. The significance level was set at p &lt;?0.05.
There were no significant differences between the antimicrobial capacity of the positive controls and the extract from the stem and fruit of B. vulgaris (p &gt;?0.05). The MIC values of the extract from the stem were significantly lower against S. sobrinus (64?μg/ml) and L. rhamnosus (128?μg/ml). The MIC value of the extract against S. mutans was significantly lower in the fruit group (64?μg/mL). The MBC value of the extract against S. sobrinus and L. rhamnosus was significantly lower in the stem group (128?μg/ml). The MBC value against S. mutans was significantly lower in the fruit group (128?μg/ml).
The results showed that CHX and B. vulgaris plant extract have similar antimicrobial activity against bacteria being associated with caries. Therefore, B. vulgaris, which shows antibacterial capacity, could be considered for further investigation as a safe, phytotherapeutic mouthwash to prevent dental caries.
The results showed that CHX and B. vulgaris plant extract have similar antimicrobial activity against bacteria being associated with caries. Therefore, B. vulgaris, which shows antibacterial capacity, could be considered for further investigation as a safe, phytotherapeutic mouthwash to prevent dental caries.In the past 40 years, China's rapid industrialization has resulted in remarkable social progress and regional economic prosperity but also has caused problems, such as excessive resource consumption and environmental pollution. Ecological efficiency is an important indicator of whether economic efficiency and environmental efficiency are balanced. Exploring ecological efficiency is vital for achieving sustainable development, as technological innovation, industrial structure upgrading, and ecological efficiency are probably related. However, there has been little research on the relationships among them. In this study, we used the super-efficiency slacks-based model (SBM) involving undesirable output to calculate the ecological efficiency of 30 provinces in China from 2008 to 2017. We found that China's ecological efficiency has risen, fallen, then fluctuated, and that there were significant spatial differences. https://www.selleckchem.com/products/danirixin.html Then, we used 3 spatial econometric models for comparative analysis, which revealed that the independent effects of technological innovation on ecological efficiency was significant and negative; however, technological innovation demonstrated a strong positive effect in tandem with industrial structure upgrading, and industrial structure always had a positive effect on ecological efficiency.